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Optimizing Lipid Extraction in Anabaena
Author(s) -
Menning Hope Deann,
Mazzer Paula Deann
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.638.1
Subject(s) - lipidome , lipidomics , anabaena , cyanobacteria , derivatization , chromatography , sonication , chemistry , biochemistry , extraction (chemistry) , mass spectrometry , botany , computational biology , biology , bacteria , genetics
Lipidomics provides a great snapshot technique to determine how the lipids work in a cell. We do not know a lot about the lipids of cyanobacteria. We set out to optimize an extraction protocol, to figure out the different lipids in a type of blue green algae, Anabaena. Our best extraction by mass is with a 10‐minute sonication step with a modified Bligh and Dyer technique. After extraction, analysis was conducted using an FTICR‐MS and derivatization of fatty acid methyl esters. We have developed the baseline lipidome for Anabaena as a starting point for future research. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .