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A novel method for insulin detection
Author(s) -
Slachtova Lenka,
Zemanova Jitka,
Sacha Pavel,
Fabre Benjamin,
Zakova Lenka,
Jiracek Jiri,
Konvalinka Jan
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.634.12
Subject(s) - insulin , diabetes mellitus , insulin receptor , drug , type 2 diabetes , chemistry , human insulin , drug target , potency , receptor , small molecule , dna , pharmacology , computational biology , in vitro , medicine , biochemistry , endocrinology , insulin resistance , biology
For some of us, insulin is a large polypeptide, for people with diabetes it is the only cure. Diabetes became a global, epidemic problem with increasing rate and health costs every year. Current treatment of type I diabetes is subcutaneously administered insulin substitution. Therefore, there is an urgent need for development of orally available insulin substitution. Here we developed a sensitive method for measurement of potency of insulin mimetics during drug development. DNA‐linked Inhibitor Antibody Assay DIANA is a sensitive, simple and nonradioactive method suitable for determination of binding constants of insulin mimetics. DIANA uses competitive inhibition of a small molecule in solution with a probe containing a ligand derivative linked to DNA. Thus, DIANA is useful method for characterization of proteinreceptor interactions, such as insulin and its receptors, as well as for high throughput screening of potential new therapeutics. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .