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Carcinogenic arecoline shows a paradoxical effect in modulating nitric oxide production and the subsequent protein S‐nitrosylation in endothelial cells
Author(s) -
Cheng BiLing,
Fu YawSyan,
Su YuanShun,
Huang Bin
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.632.4
Subject(s) - arecoline , nitric oxide , s nitrosylation , chemistry , enos , western blot , carcinogenesis , microbiology and biotechnology , biochemistry , pharmacology , cancer research , medicine , biology , nitric oxide synthase , cysteine , receptor , muscarinic acetylcholine receptor , enzyme , organic chemistry , gene
Arecoline exhibits a carcinogenic tone and is remarkable for the refreshing effect due to the increase of vasodilation and blood flow. Therefore, if arecoline can promote the production of nitric oxide (NO) and the subsequent protein S‐nitrosylation in endothelial cells (ECs) is interesting. Treating arecoline increased the phosphorylation of eNOS Ser1177 . NO molecular was observed by a fluorescent probe FA‐OMe followed with the measurement of flowcytometry. Arecoline treatment up‐/down‐regulated 914/647 proteins identified by showing by iTRAQ 114/115 labeling and LC‐MS/MS. IPA analysis indicated that these proteins were implicated in eNOS signaling. By using biotin switch, 224/159 S‐nitrosoproteins were up‐/down‐regulated by arecoline. The increased S‐nitrosylation level of chaperonin and the decreased S‐nitrosylation level of calnexin were verified by western blot. Our results suggest a paradoxical effect of arecoline in producing NO and therefore maintains endothelial homeostasis, even though the toxicological tone in carcinogenesis remains concerned. Support or Funding Information TB0357 from Ministry of Education, Taiwan This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .