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Interaction between Grx4, Fep1 and Fra2 regulates low iron response in S. pombe
Author(s) -
Gupta Malini,
Outten Caryn
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.631.19
Subject(s) - chemistry
Iron associates with proteins involved in critical biological functions such as electron transfer, oxygen transport, DNA metabolism and repair, and photosynthesis. Intracellular iron regulation is thus critical since both iron excess and iron deficiency have deleterious physiological consequences. In the model eukaryote S. pombe , Fep1 is a GATA type transcriptional repressor active under iron replete conditions. Previous in vivo results suggest that the cytosolic glutaredoxin Grx4 acts as an inhibitory partner of Fep1 under iron starvation conditions. Also in the absence of Fra2, Fep1 has been shown to be constitutively bound to target promoters irrespective of iron levels in the cell. The significance of Fra2 as a co‐inhibitory partner of Fep1 with Grx4 is yet to be established. In S. pombe , interaction between Grx4‐Fep1and Fra2‐Fep1 has been reported in vivo . Here, we are using in vitro approach to characterize the Fe‐dependent interactions between Fep1, Grx4 and Fra2. We have been able to co‐purify Fep1, Grx4, Fra2 as a complex and will be using biochemical and spectroscopic tools to probe the molecular details of this interaction. Support or Funding Information R01 grant (GM086619)R35 grant (GM118164)Working model for interaction between Grx4, Fep1 and Fra2This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .