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Transfer RNA post‐transcriptional modifications of Bacillus subtilis
Author(s) -
Psihountas Christina,
Jora Manasses,
Limbach Patrick A
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.630.7
Subject(s) - bacillus subtilis , transfer rna , domestication , strain (injury) , nucleoside , rna , biology , genetics , chemistry , biochemistry , bacteria , computational biology , gene , anatomy
Transfer Ribonucleic Acids (tRNAs) have more posttranscriptional modifications to nucleosides than other species of RNA.1 It has been found that when bacteria are analyzed at different stages of a growth curve, changes in these nucleoside modification identities or amounts exist.2 Similarly, nucleoside modification profiles are dependent on growth media.3 Here we are examining whether domestication of Bacillus subtilis lead to differences in nucleoside modification profiles for tRNAs. We have compared tRNA modifications from two different strains: (i) a domesticated lab strain 168, the most widely studied strain, and (ii) strain NCIB 3610, a Marburg strain isolate. NCIB 3610 is reported in literature to be “wilder” than strain 168.4 We are using strain 168 as the B. subtilis model against which we can evaluate how domestication may impact the types and quantities of tRNA modifications. Our experimental approach is to use liquid chromatography tandem mass spectrometry (LC‐MS/MS). Once nucleosides are identified and quantified, we will then compare those results to the genetic variations arising between the domesticated and undomesticated strains to understand how such modification changes may arise. Support or Funding Information The National Science Foundation (CHE 1507357) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .