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Modulation of gene expression by Salvia hispanica seed extract in MC3T3 cells
Author(s) -
Mendez Daisy May,
Rey Maximo,
Hernandez Tomas,
Vel Jaysonn,
Sanchez Diego,
Nair Saraswathy,
Banu Jameela
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.619.6
Subject(s) - osteopontin , osteocalcin , alkaline phosphatase , wnt signaling pathway , osteoblast , gene expression , rna , microbiology and biotechnology , complementary dna , bone sialoprotein , collagenase , bone remodeling , gene , biology , chemistry , signal transduction , endocrinology , biochemistry , enzyme , in vitro
Postmenopausal bone loss weakens bones and make them fragile and easily susceptible to fractures. Available therapies have severe side effects increasing the need for new therapeutic agents that can be effective in protecting bone loss and have less‐side effects. Recently, Salvia hispanica (SH) is reported to have several health benefits and high calcium levels. Our studies using a rodent model for postmenopausal bone loss showed bone protective properties, we decided to determine the influence of SH seed extract on osteogenic gene expression. We cultured MC3T3 cells, differentiated them into osteoblast and treated them with Salvia hispanica (SH) seed extract at optimal concentration. Cells were harvested, for gene expression studies, RNA was isolated using Purezol RNA isolation reagent (BioRad). cDNA was synthesized using standard methods. The cDNA was assessed for the expression of genes related to osteogenesis in real‐time RT‐PCR using gene specific primers and Taqman assays. Alkaline phosphatase, BMPr2, osteocalcin, osteopontin, DCAT (inhibitor of beta catenin – wnt signaling pathway) and Type 1 collagenase. Alkaline phosphatase increased (58%) when treated with SH seed extract while type 1 collagen (18%), Bgl (19%), BMP2r (80%) decreased in the SH group. The expression of DCAT1, an antagonist of b‐catenin, also significantly decreased (75%) after treatment with SH. This suggests that SH may act via the Wnt signaling pathway to modulate bone metabolism. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .