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Invitro insulinotropic actions of various extracts of Calosia argentea leaves
Author(s) -
Ogunmodede Oluwaseyi Samson,
Oseni Saheed Oluwasina,
Adenmosun Olumide Olaseni,
Mbagwu Smart Ikechukwu
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.610.13
Subject(s) - incubation , dichloromethane , cytotoxicity , extraction (chemistry) , chemistry , acetone , lactate dehydrogenase , trypsin , chromatography , insulin , biochemistry , in vitro , enzyme , solvent , biology , endocrinology
This study investigated the effects of Calosia argentea obtained by different extraction methods on the insulin‐release and cytotoxicity on clonal pancreatic beta cells. Pulverized Calosia argentea leaves were sequentially extracted with dichloromethane, acetone, ethylacetate and water. Clonal pancreatic beta cells, BRIN‐BD11, were grown in an atmosphere of 5% CO2 and 95% air in RPMI‐1640. Insulin and LDH assays were carried out using Krebs Ringers Bicarbonate buffer. Insulin concentration was measured by radioimmunoassay and LDH release was assessed using a commercially available non‐radioactive assay kit (Promega, UK). Aqueous extraction of Calosia argentea produced the best yield (361 mg/20kg powder). Acute incubation of BRIN‐BD11 cells with different extracts of Calosia argentea produced a concentration‐dependent stimulation of insulin‐release at concentrations ≥10 μg/ml. The aqueous extract of Calosia argentea produced the best insulinotropic effect, with a maximum stimulation of 347% (P<0.001) of basal rate at 100μg/ml and the lowest stimulating concentration of 0.1 μg/ml. Dichloromethane and acetone extracts produced similar stimulatory effects (130% of basal rate, 100μg/ml, P<0.01). Insulin‐releasing effects of the four extract were not associated with beta cell cytotoxicity. The release of the cytosolic enzyme, lactate dehydrogenase (LDH), due to damaged plasma membrane was used as a marker of cytotoxicity in this study. Significant (P<0.001) release of the enzyme was observed in incubations involving DMSO (40% v/v). However, incubation with the different extracts of Calosia argentea produced no significant release of LDH. These results showed that extracts of Calosia argentea possess insulinotropic effects and encourage further studies to uncover the physiological mechanism involved. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .