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Adhesion of Human Umbilical Vein Endothelial Cells (HUVEC) on PTFE Material Following Surface Modification by Low Temperature Plasma Treatment
Author(s) -
VIG KOMAL,
Swain Kendra,
Mlambo Takura,
Baker Paul,
Tucker Bernabe,
Thomas Vinoy,
Vohra Yogesh K
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.603.3
Subject(s) - intimal hyperplasia , umbilical vein , fibronectin , viability assay , chemistry , contact angle , human umbilical vein endothelial cell , adhesion , surface modification , endothelial stem cell , biomedical engineering , cell , medicine , materials science , in vitro , biochemistry , composite material , organic chemistry , smooth muscle
Cardiovascular disease (CVD) is the no. 1 killer in the world, and is responsible for >17.3 million deaths every year 1 . Bypass surgery is the most effective treatments for CVD and often use the autologous vein graft. More recently vascular grafts made of materials such as Dacron and ePTFE that mimic the native vessel wall 2–3 , have shown great potential in bypass surgery. However, their use in small diameter grafts (<6 mm) is limited due to intimal hyperplasia and thrombosis. The current study is aimed to modify the intimal graft surface with low temperature plasma (LTP) to increase the cell attachment/viability and proliferation. The scaffolds were treated with LTP using Harris Plasma Cleaner system with air as the feed gas for 30 sec at 45W (HI setting). X ray photoelectron spectroscopic analyses and contact angle wettability studies confirmed the introduction of oxygenated functionalities on the surface and enhanced hydrophilicity due to the improvement in oxygen content ~1 in the graft surface from LTP air plasma. LTP treated scaffolds were further modified with fibronectin and collagen by dipping method. Cell studies by microscopic and metabolic assays indicated that HUVEC cell viability increased in LTP treated scaffolds especially when treated with protein. Scaffolds treated with fibronectin or collagen had cells viable for a week compared to untreated samples. MTT results validated the improved number of metabolically active viable cells. Work is under progress to improve cell viability on these scaffolds and investigate gene expression profiles of the cultured cells on these scaffolds. Support or Funding Information This work is supported by the NSF EPSCoR RII‐Track‐1 Cooperative Agreement OIA‐1655280. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .