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Protective effects of Dianthus superbus on Diabetic renal dysfunction in in vivo and in vitro models
Author(s) -
Yoon Jung Joo,
Son Chan Ok,
Kim Hye Yoom,
Lee Hyeon Kyoung,
Lee Yun Jung,
Yang Sun Nye,
Lee Ho Sub,
Kang Dae Gill
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.567.18
Subject(s) - medicine , endocrinology , diabetic nephropathy , biology , fibrosis , kidney
Glomerular fibrosis is caused by accumulation of extracellular matrix proteins in the mesangial interstitial space resulting in fibrosis manifested by either diffuse or nodular changes. Dianthus superbus belongs to the Caryophyllaceae family and has been used in traditional medicine as a diuretic, a contraceptive, and an anti‐inflammatory agent. The aim of this study was to investigate the effects of Dianthus superbus on glomerular fibrosis and renal dysfunction causing diabetic nephropathy in human renal mesangial cells and db/db mice. In diabetic db/db mice models, Dianthus superbus were administrated to db/db mice (n=8) to 10 or 50 mg/kg/day concentrations. The experiment was measured for 8 weeks following Dianthus superbus treatment respectively. As a result, Dianthus superbus significantly ameliorated blood glucose, insulin, HOMA‐IR and HbA1c of diabetic index. Dianthus superbus decreased albumin excretion, creatinine clearance and plasma creatinine. Dianthus superbus ameliorated kidney injury molecules‐1 of kidney function index and C‐reactive protein of inflammation marker. In addition, Dianthus superbus reduced the PAS positivity stain intensity and basement membrane thickening in glomeruli of diabetic nephropathy model. Dianthus superbus suppressed TGF‐β/Smad signaling pathway. Collagen type IV, glomerular fibrosis biomarker, was significantly decreased by Dianthus superbus. Additionally, Dianthus superbus pretreatment attenuated inflammatory factors expression and translocation of nuclear factor kappa B (NF‐κB) in Angiotensin II (Ang II)‐stimulated mesangial cell. These findings suggest that Dianthus superbus has protective effect against renal inflammation, fibrosis and oxidative stress. Therefore, Dianthus superbus may be potential therapies targeting glomerulonephritis and glomerulosclerosis leading to diabetic nephropathy. Support or Funding Information This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .