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Hippocampal neural stem cells undergo fate switch following intermittent hypoxia
Author(s) -
Khuu Maggie A.,
Nallamothu Thara,
Garcia Alfredo J.
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.551.20
Subject(s) - neurogenesis , sox2 , neural stem cell , hippocampal formation , dentate gyrus , neuroscience , progenitor cell , biology , intermittent hypoxia , precursor cell , stem cell , microbiology and biotechnology , medicine , obstructive sleep apnea , cell , embryonic stem cell , genetics , gene
Adult neurogenesis is a phenomenon widely recognized as a process supporting neurophysiology of the hippocampus. We recently reported that 30 days of intermittent hypoxia (IH), a principal consequence for sleep apnea, stimulates the expansion of hippocampal neural stem cells (NSCs), yet suppresses the generation of adult‐born neurons. This study investigates how IH impacts NSCs by using a shorter duration of IH. We hypothesize that 10 days of IH (IH10) redirects the course of development of NSCs. We examined how NSCs in the neurogenic niche of the dentate gyrus are affected by IH10. Immunohistochemical analysis revealed that the number of NSCs (Sox2+ cells) was not increased following IH10, yet appears to increase in quiescent NSCs (i.e., Sox2+ cells that co‐express the astrocytic marker, S100b, and do not develop into adult‐born neurons). Fewer NSCs were observed transitioning into intermediate neuronal progenitors (INPs, SOX2+/TBR2+ cells) and a reduction in the total number of INPs (TBR2+ cells) was also noted. These experiments suggest that the impact of IH on cells involved with adult neurogenesis is cell‐type specific. Our findings provide further support that untreated sleep apnea may impact the fate of pluripotent cellular constituents in the central nervous system. Support or Funding Information This work was supported by NIH R01 NS10742101 (AJG) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .