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Oxidative Stress and Inflammation in Wound Repair: Molecular and Cellular Mechanisms
Author(s) -
Pianissoli Lícia Gobeti,
Silva Julia Paiva Rodrigues,
Penteado Elis Arantes,
Lopes Diana Ramlow Coelho,
Mengal Vinicius Franskoviaky
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.542.3
Subject(s) - inflammation , oxidative stress , wound healing , nitric oxide synthase , medicine , catalase , nitric oxide , immunology , bioinformatics , biology
Oxidative stress and inflammation are the critical factors attributed with delay in wound repairing process. Experimental studies established the dogma that inflammation is essential to the establishment of cutaneous homeostasis following injury, and in recent years information about specific subsets of inflammatory cell lineages and the cytokine network orchestrating inflammation associated with tissue repair has increased. Recently, this dogma has been challenged, and reports have raised questions on the validity of the essential prerequisite of inflammation for efficient tissue repair. Indeed, in experimental models of repair, inflammation has been shown to delay healing and to result in increased scarring. The main focus of present study was to explore the effect of oxidative stress and inflammation in the wound healing in patients with pressure injuries. The study was performed in patients in the medical clinic, from a general hospital, located in Espirito Santo‐Brazil. Weekly swab collection was performed in order to follow the healing process and therapeutic response. Molecular biology analyzes were performed to measure the presence increase, or time‐dependent reduction related to oxidative stress and inflammatory process. Our data show that pressure injuries in stages 2 and 3 of difficult healing present reduced of antioxidant enzymes, such as SOD and Catalase, as well as an increase in oxidant and inflammatory enzymes. We also evaluated the changes in inducible nitric oxide synthase (iNOS) and other inflammatory enzymes using western blot. Our results showed that, in pressure injuries, there is higher level of iNOS, inflammatory and oxidants enzymes. iNOS is linked to increased inflammation, leading to prolonged wound healing. Our results suggest that treatments that aim to reduce the local inflammatory process and consequently the oxidative stress can improve the healing process, as well as make the tissue repair process easier. Support or Funding Information This research was supported by a CNPq research grant. This study was funded by Fundação de Amparo a Pesquisa do Espirito Santo (FAPES‐Brazil) and Conselho Nacional de Desenvolvimento Científico e Tecnológico. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .