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The Effect of Royal Jelly on Satellite Cell Activation in Aged Monkey Muscle Cells
Author(s) -
Sato Shuichi,
Boudreaux Seth P,
Bellar David
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.539.1
Subject(s) - sarcopenia , skeletal muscle , life expectancy , progenitor cell , satellite , biology , stem cell , cell , cell growth , immunology , microbiology and biotechnology , physiology , medicine , endocrinology , andrology , population , biochemistry , environmental health , aerospace engineering , engineering
While global average life expectancy has been consistently increasing in recent year, a gap between healthy life expectancy and the average life expectancy has not been filled yet. Sarcopenia, which is characterized as age‐associated decline of skeletal muscle mass, is one of the most common causes of functional decline and loss of independence, impeding healthy life style in the elderly. Satellite cells are myogenic progenitors and sarcopenia is predicted to be the result of reduced satellite cell number and/or function that leads to a loss of capacity for skeletal muscle mass maintenance. Royal jelly (RJ) produced by worker honeybees is known to have an antioxidant effect and a previous study demonstrated that royal jelly treatment was of benefit on revitalizing aged satellite cells in vitro and muscle regenerative capacity after injury in mice. However, the mechanism by which RJ stimulates the activation of satellite cells is not known. We aimed at further investigating if RJ can attenuate the loss of satellite cell number and/or function by improved satellite cell proliferation using the cells isolated from aged nonhuman primates, which can closely simulate humans. Muscle biopsy from quadriceps was performed then primary muscle cells were isolated from untreated rhesus macaques (young: u up to 10 years old, aged: 18~20 years old). The cells were cultured in either 6‐well or 12‐well plates in growth medium (F‐12 with 10% FBS, 1% Pen/Strep, and hEGF) at least 48 hours. For treatment group, protease‐treated RJ (Institute for Bee Products & Health Science, Okayama, Japan) was dissolved in PBS and added to the culture medium at different concentrations (250, 500, or 1000 μg/mL). After 48 hours of treatment, the cells were harvested and biochemical analyses were conducted. Flow cytometry analysis revealed that the levels of satellite cell activation/proliferation were decreased in aged monkey muscle cells compared to those of young ones (17.0±16.3% vs. 87.4±3.7%, respectively, p<0.05). qRT‐PCR analysis showed an elevation of mRNA levels of PAX7 (1.00±0.14 for control vs. 1.76±0.43 for RJ treatment group, p<0.05) in aged cells with RJ treatment. Enzyme activity assay demonstrated that 48‐hour RJ treatment increased catalase enzymatic activity by 24% (p<0.05). However, the levels of PAX7+/MyoD+ cells, indicative of activated/proliferating satellite cells, were comparable between two groups, which were confirmed by flow cytometry. In conclusion, RJ administration may stimulate satellite cell activation by moderately reducing oxidative stress. Support or Funding Information Supported by Yamada Research Grant (No. 0221). This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .