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Preliminary Data on the Effect of High Laminar Shear Stress on TNFR1 and Adaptor Proteins Expression in Endothelial Cells
Author(s) -
Aldokhayyil Maitha,
Gomez Dulce,
Brown Michael
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.527.13
Subject(s) - tumor necrosis factor alpha , umbilical vein , receptor , cytokine , chemokine , signal transducing adaptor protein , inflammation , microbiology and biotechnology , monocyte , chemistry , immunology , medicine , biology , in vitro , biochemistry
Background Tumor Necrosis Factor (TNF) is an inflammatory cytokine that targets the vascular endothelial cells and initiates a cascade of events that leads to endothelial dysfunction. TNF‐induced activation of NF‐κB and MAP kinases increases the expression of adhesion molecules and chemokines that cause leukocytes recruitment and activation consequently amplifying inflammation. Evidence suggests that high laminar shear stress (HLSS) mitigates inflammation and induces atheroprotective effects. Thus, the purpose of this preliminary study was to investigate the effect of HLSS on the gene expression of TNF Receptor 1 (TNFR1) and two associated adaptor proteins, TNF receptor‐associated factor‐2 (TRAF2) and lymphocyte adaptor protein (Lnk). Methods Human umbilical vein endothelial cells (HUVECs) from two donors were cultured and incubated with TNF (10 ng/mL) for 4hr, exposed to HLSS (20 dynes/cm 2 ) for 24hr, or pre‐exposed to HLSS for 24hr and then incubated with TNF (10 ng/ml). The experiment was repeated 2–3 times. Results We found a significant decrease in TNFR1 expression in response to TNF incubation ( p = .001, Fig. 1). TNFR1 expression was further decreased with HLSS ( p = .04, Fig. 1). TNF incubation and HLSS also significantly reduced TRAF2 expression by about 50% ( p <.01, Fig. 2). In response to HLSS, Lnk expression was significantly reduced ( p <.001, Fig 3). However, this reduction was blunted by TNF incubation for 4hr ( p = .02 Fig. 3). Conclusion The results suggest that HLSS acts as an exercise memetic to significantly affect TNF signaling proteins. HLSS may help as a novel preventive and therapeutic approach to modulate TNF signaling and improve vascular function. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .