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Integrin‐mediated Vasoconstrictor Function Declines with Age in Skeletal Muscle Resistance Arteries
Author(s) -
Shin Song Yi,
Trache Andreea,
Woodman Christopher
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.518.4
Subject(s) - medicine , vascular smooth muscle , vasoconstriction , phenylephrine , endocrinology , contractility , vascular resistance , skeletal muscle , smooth muscle , hemodynamics , blood pressure
Vasoconstrictor responses decline with age in skeletal muscle resistance arteries. The mechanism for functional regulation of resistance artery contractility is not fully understood but has been proposed to involve impaired integrin signaling in vascular smooth muscle. Objective The purpose of this study was to test the hypothesis that RGD binding integrin‐mediated constrictor responses decline with age in skeletal muscle resistance arteries. Methods Soleus muscle feed arteries (SFA) were isolated from young (4 mo) and old (24 mo) male Fischer 344 rats. SFA were cannulated with glass micropipettes and pressurized to 90 cm H 2 O for assessment of vasoconstrictor function. Vasoconstrictor responses were assessed using an a1 selective adrenergic agonist (phenylephrine; PE). To test the role of integrin signaling, constrictor responses were assessed in the absence or presence of an integrinblocking peptide (RGD) or a control peptide (RGE). Results Constrictor responses to PE were significantly impaired in old SFA relative to young SFA. In the presence of the RGD soluble peptide, constrictor responses to PE were significantly reduced in young SFA such that the young SFA responded like control (no treatment) old SFA. In the presence of the RGD peptide, constrictor responses to PE were further reduced in old SFA. Constrictor responses to PE were not altered by RGE in young or old SFA. Conclusion The results of this study indicate that RGD binding integrin‐mediated constrictor function declines with age in SFA. Support or Funding Information Research support: National Institute on Aging, Sydney and J.L. Huffines Institute of Sports Medicine and Human Performance. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .