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Bifidobacterium breve and Lactobacillus rhamnosus Attenuate the Inflammatory Mediators Secretion in a Human Bronchial Epithelial (BEAS) Cells Culture Stimulated with Cigarette Extract
Author(s) -
Aimbire Flavio,
Miranda Munique,
Sá Ana Karolina,
Carvalho Jose Luis
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.516.6
Subject(s) - lactobacillus rhamnosus , bifidobacterium breve , copd , probiotic , chemokine , medicine , cxcl1 , interleukin 8 , secretion , immunology , tumor necrosis factor alpha , cytokine , bifidobacterium , microbiology and biotechnology , inflammation , biology , lactobacillus , genetics , bacteria
Abnormal inflammatory response in lung tissue is the main characteristic of chronic obstructive pulmonary disease (COPD), mainly triggered by exposure to cigarette smoke. COPD is one of the major causes of morbidity and mortality, with a great socioeconomic impact worldwide. Despite the importance of the disease and its impact, existing treatment still presents limitations and the presence of several side effects. Therefore, the search for alternative therapies is extremely relevant and the use of probiotics in the treatment of COPD has been highlighted, due to its immunomodulatory potential in chronic inflammatory diseases. Our study proposes to evaluate the role of Bifidobacterium breve (Bb) and Lactobacillus rhamnosus (Lr) probiotics on the in vitro inflammatory profile of cytokine released after stimulation of bronchial epithelial cells (BEAS) with cigarette smoke extract. We stimulated human derived BEAS culture with 2.5% cigarette extract, produced from the burning of 1 cigarette, whose smoke was incorporated into the culture medium (10 ml) through a vacuum pump (−11Kpa). After four hours, Lr or Bb was added to the BEAS culture. 24 hours after probiotic administration, the supernatant was collected to be measured by ELISA. The extract was sufficient to stimulate the cell lineage. Both Lr and Bb were efficient in reducing levels of IL‐6, IL‐1β, TNF‐α, CXCL1, CXCL5, CXCL8 and CXCL9, while increasing IL‐10 and TGF‐β levels. The probiotics proposed modulate the synthesis of pro‐inflammatory mediators, reducing the concentration of cytokines and chemokines secreted in the BEAS culture supernatant. Support or Funding Information FAPESP This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .