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Role of probiotics Bifidobacterium breve and Lactobacillus rhamnosus on inflammation lung in an experimental model of chronic obstructive pulmonary disease
Author(s) -
DE SÁ FIALHO ANA KAROLINA CARVALHO,
MIRANDA MUNIQUE TOSTES FERREIRA,
CARVALHO JORGE LUIS COSTA,
BRITO AURILEIA APARECIDA,
ALBERTINI REGIANE,
AIMBIRE FLÁVIO
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.516.4
Subject(s) - medicine , bronchoalveolar lavage , copd , lactobacillus rhamnosus , lung , inflammation , immunology , probiotic , biology , bacteria , genetics
Chronic pulmonary obstructive disease (COPD) is defined as an abnormal inflammatory response of the lungs to noxious particles or gases, mainly cigarette smoke. Thus, new therapeutic approaches are of unquestionable relevance. Without a specific treatment for COPD patients the use of probiotics via supplementation of diet can be a promising target. This project aims to investigate the beneficial effects of Bifidobacterium breve (Bb) and Lactobacillus rhamnosus (Lr) probiotics in lung inflammatory process in mice in a model of lung emphysema. Methods This study was approved by the Ethics Committee of the UNIFESP. The COPD was induced by cigarette smoke inhalation of 14 cigarette per day, twice a day, 7 times/week during 60 days in C57Bl/6 mice. The mice were treated with Lr and Bb at the same time. The pro‐inflammatory mediators as IL‐6, TNF, IL‐1 β, CXCL1, CXCL8, CXCL10, KC, CXCL9, CXCL11 and anti‐inflammatory as IL‐10 in bronchoalveolar lavage fluid (BALF) were measured by ELISA. The expression of mRNA of the MMP9 and MMP12, NF‐κB, STAT3 and TLR 2,4 and 9 in lung were analyzed by quantitative RT‐PCR. The NF‐κB was also analyzed by immunolocalization. The lung tissue was used for histological and morphometric analyzes. Results Bb and Lr attenuated the cellularity in BALF and reduced the pro‐inflammatory cytokines and inversely increased the anti‐inflammatory. Also, the probiotics reduced the expression of MMP9 and 12, NF‐κB, STAT3 and TLR 2,4 and 9 in lung. Then, the probiotic also changed the airway remodeling (inflammatory infiltrate, alveolar enlargement, collagen, and elastic fibers). Conclusion This study points out that the probiotics decreased the cellularity on the BALF and the expression of the main transcription factors as a consequence they also reduced pro‐inflammatory cytokines and inversely increase anti‐inflammatory. These results contribute to the understanding of Bb and Lr modulator activities. Support or Funding Information Financial Support: Foundation for Research Support of the State of São Paulo (FAPESP) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .