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Antiproliferative Natural Product Superstolide/ZJ‐101 Affects Cellular Adhesion in 3D Spheroid Model
Author(s) -
Sanchez Phillip R,
Head Sarah A,
Jin Zhendong,
Liu Jun O
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.504.3
Subject(s) - natural product , phenotype , cell adhesion , cytotoxic t cell , adhesion , spheroid , chemistry , cell culture , phenotypic screening , in vitro , viability assay , cancer research , cell , microbiology and biotechnology , biology , biochemistry , genetics , gene , organic chemistry
The natural product known as Superstolide/ZJ‐101 has anti‐proliferative activity across a multitude of human cancer cell lines with an IC50 in the range of 30–100 nanomolar. Structure activity relationships have improved this activity to the single‐digit nanomolar range in 2D viability assays. Additionally, phenotypic assays have identified a strong cytostatic rather than cytotoxic effect. Recently ZJ‐101 has been tested in a 3D spheroid model of triple‐negative breast cancer to reveal a novel phenotype of cellular adhesion disruption. This effect has also been observed in 2D culture, indicating that both cell‐cell and cell‐substrate adhesion are similarly affected. Taken together, the anti‐adhesion phenotype could suggest a new type of cytostatic therapeutic which dissolves intractable tumors and prevents their migration, invasion and metastasis. Support or Funding Information NIH T32 Pharmacological Sciences Training Grant This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .