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Cellulose hydrogels as delivery vehicle of immunomodulatory mesenchymal stem cells
Author(s) -
Flores Andrea Sofia,
Gonzalez Keishla,
Domenech Maribella
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.494.2
Subject(s) - mesenchymal stem cell , self healing hydrogels , cellulose , spheroid , secretion , chemistry , microbiology and biotechnology , in vitro , stem cell , immunology , biophysics , biomedical engineering , medicine , biology , biochemistry , organic chemistry
Mesenchymal stem cells (MSCs) are cells that have the capacity of differentiation and specialization in skeletal tissues, and more recently immunomodulatory properties. Upon stimulation MSCs secrete immunosuppressive factors that can benefit patients with inflammatory conditions. To prolong therapeutic benefit of MSCs following implantation, delivery strategies that provide protection from external damage are needed. In this study, we evaluated a cellulose gel as a vehicle for encapsulation and delivery of MSCs. Cellulose is biocompatible and non‐degradable in humans, and the use of hydrogels can control the release of anti‐inflammatory factors. To determine the effectiveness of using cellulose hydrogels as a delivery vehicle and establish the relationship between MSCs and cellulose, MSCs were cultivated into three‐dimensional spheroids in cellulose matrixes of 0.3% and 0.5%. Proliferation was measured by spheroid growth over the course of 10 days and was then tested for the secretion profile of its main immunomodulatory factor, indoleamine‐pyrrole 2,3‐dioxygenase (IDO), after stimulation with IFN‐γ +/−. These results were compared to two‐dimensional controls, where higher secretion of IDO was expected in the three‐dimensional models. Multiple spheroids were formed in each concentration instead of one large spheroid. Proliferation was directly proportional to cellulose concentration, although the difference in spheroid growth between both concentrations was considered insignificant. These results show that cellulose based materials can be used as a matrix for in‐vitro studies and as a delivery vehicle for immunomodulatory MSCs. Support or Funding Information Financial support from the National Institute of Health RISE program under grant number 1R25GM127191‐01 This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .