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Differential Splicing of Perilipin 5
Author(s) -
Jordan Spencer P,
Tansey John T
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.490.6
Subject(s) - perilipin , rna splicing , lipid droplet , intron , biology , alternative splicing , lipolysis , microbiology and biotechnology , biochemistry , gene , messenger rna , adipose tissue , rna
Included among the most common diseases in the U.S., are metabolic diseases such as type II diabetes mellitus and nonalcoholic fatty liver disease. Understanding these diseases requires an understanding of neutral lipid storage and metabolism. Perilipins are a family of five conserved proteins that function in the regulation of neutral lipid metabolism. Of the perilipins, perilipin 5 is the most recently discovered and studied protein in the family. Perilipin 5 is a major regulator of lipolysis and is expressed in highly oxidative tissue such as heart, oxidative muscle, and fasting liver. It is primarily found localized to the surfaces of lipid storage droplets, but it can also be found in the cytosol. Alternative splicing has been observed in perilipins 1 and 3, resulting in several other proteins with various functions as these proteins lack domains which have the functions of the full‐length protein. We have identified a truncated form of perilipin 5 we have termed perilipin 5b using western blotting. Reverse transcriptase PCR indicate that this truncation results from a read through of one of several different introns, most likely the intron between introns 7 and 8. cDNA BLAST searches also provide evidence of this splice variant. Collectively, these data suggest that like other members of the family, perilipin 5 undergoes differential splicing resulting in shortened protein products with short, unique carboxy terminal tails. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .