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Interactions of Perilipin 5 with Cytoskeletal Elements
Author(s) -
Austin Rachel L,
Tansey John T
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.490.1
Subject(s) - perilipin , lipid droplet , cytoskeleton , microbiology and biotechnology , cytoplasm , actin , lipid metabolism , mitochondrion , biology , chemistry , lipolysis , biochemistry , cell , adipose tissue
Cardiovascular diseases, obesity, and diabetes are disease that are affecting the American population at alarming rates. Central to these diseases is lipid metabolism and lipid storage. Perilipin 5 is a protein found on the surface of lipid droplets within cells, but has also been found within the cytoplasm, mitochondria, and nucleus, and is implicated in the mobilization of lipid droplets. This mobilization is required to associate triglycerides with mitochondria, where beta oxidation occurs. Cellular trafficking of lipids is also aided by cytoskeletal elements – like actin filaments. These filaments provide a mode for transportation for molecules within the cell. We hypothesize perilipin 5 will interact with cytoskeletal elements. To test this, we are conducting immmunofluorescence on cultured cells expressing perilipin 5 and stained with antibodies against perilipin 5 and fluorescent actin filaments. Evidence of this interactions will add more information about the role of Perilipin 5 in fatty acid oxidation, the dysfunction of lipolysis, and its associated diseases. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .