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Knock‐on effects of gestational diabetes in offspring learning and anxiety without influence of oxidative stress: Effects dependent on sex and age
Author(s) -
HuertaCervantes Maribel,
PeñaMontes Donovan,
LópezVázquez Miguel Angel,
OlveraCortés María Esther,
CortésRojo Christian,
ManzoAvalos Salvador,
SaavedraMolina Alfredo
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.487.8
Subject(s) - offspring , endocrinology , oxidative stress , medicine , morris water navigation task , gestation , elevated plus maze , streptozotocin , open field , pregnancy , gestational diabetes , fetus , diabetes mellitus , hippocampus , prenatal stress , lipid peroxidation , anxiety , biology , genetics , psychiatry
Gestational diabetes (DG) is an impairment of glucose metabolism during pregnancy, an increment of blood glucose in the intrauterine milieu results in abnormal fetal development and an increase in the risk of developing metabolic disorders as type 2 diabetes mellitus, neurodegenerative diseases and behavioral abnormalities in the offspring. In addition, increased oxidative stress has been reported in the offspring of rats under experimental GD in several brain regions at early ages. However, the insult in the offspring behavior in young and adult stages and oxidative stress in cerebral regions as hippocampus and cortex remains unclear. The aim of this study was to evaluate the effect of gestational diabetes in female and male offspring on behavior and oxidative stress in juvenile and adult stages. Twelve Wistar female rats were place mate with a control male. Gestation pregnant rats (day 7) were divided randomly into two groups: Gestation control rats (GC) and gestational diabetic rats (GD). GD rats were injected with a single intraperitoneal injection of a low dose of streptozotocin (STZ), only rats exhibiting blood glucose levels 140–300 mg/dL were considered for the study. Two‐months old rats (juvenile) and six‐months old rats (adult) male and female offspring from GC and GD were employed in this work. We evaluate emotionality and anxiety in the elevated plus maze, and open field, the Morris water maze was employed to evaluate spatial learning, and the radial maze for short‐term working memory. Oxidative stress was evaluated measuring the oxidative stress biomarkers (lipid‐peroxidation and GSH:GSSG ratio) and reactive oxygen species production (ROS) in homogenate of hippocampus and cortex. We found an impairment in spatial learning of offspring DG female rats, and a low anxiety level in offspring DG male on juvenile stage, this disturbs did not remain at adult stage. On the other hand, we have not found changes in lipid‐peroxidation and GSH:GSSG ratio and ROS production. In conclusion, these results suggest that GD affects learning and anxiety behavior without the involvement of oxidative stress in the juvenile stage of gestational diabetes offspring. Support or Funding Information Financial support grant from CONACYT (MHC), and Coordinación de Investigación Científica (UMSNH) (2.16 to ASM). This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .