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Cell morphology changes in 3T3‐L1 cells grown in monolayer and treated with cinnamon extract
Author(s) -
Baxendell Kayla,
Aulthouse Amy,
Stockert Amy
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.487.4
Subject(s) - cytochalasin b , cell , cell culture , staining , morphology (biology) , cytochalasin , biology , chemistry , microbiology and biotechnology , biochemistry , cytoskeleton , genetics
Cinnamon has long been used medicinally and has gained attention more recently due to its ability to decrease blood glucose values in type 2 diabetes. Research has suggested potential targets in the cell responsible for the metabolic effects observed with cinnamon, however, a collective mechanism of action is still unknown. This study demonstrates morphological changes observed in 3T3‐L1 cells grown in monolayer and treated with cinnamon extract that mimic cell differentiation observed in some three‐dimensional models. Data is presented that demonstrates changes in cell shape with increasing concentrations of cinnamon extract. Evidence of lipid accumulation is visualized by oil red O staining as early as 0.5 weeks following cinnamon treatment. Monolayer cells exhibit round morphology when treated with cytochalasin D, an actin polymerization inhibitor. Together these data suggest that one potential metabolic target of cinnamon extract may result to alter actin polymerization leading to cell rounding. The morphological change from flat to round cells potentially stimulates changes in gene expression that lead to differentiation. Future work will determine the extent of differentiation observed in the cinnamon treated cells compared to those grown with cytochalasin D and those grown in three‐dimensional culture. This study outlines possible unique therapeutic approachs for the alteration of cell differentiation that could result in desired metabolic changes. Support or Funding Information Ohio Northern University Raabe College of Pharmacy and the Department of Biological and Allied Health This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .