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The Effect of Manuka Honey's effect on Ampicillin‐Resistant Escherichia coli HB101 K‐12 Compared to other Antibiotics
Author(s) -
Guadarrama David
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.483.8
Subject(s) - manuka honey , antibiotics , ampicillin , antimicrobial , microbiology and biotechnology , antibiotic resistance , streptomycin , escherichia coli , biology , medicine , biochemistry , gene
Antibiotics have been considered as one of the greatest achievements in the medical field and its use has helped save countless lives from dangerous pathogens. However, the dependency of antibiotics now holds a great risk for the future of society. Through the overuse of antibiotics by developing and underdeveloped countries, the chances of superbugs developing resistance have become higher. By limiting the use of antibiotics or finding new alternatives, it can reduce the chances that naturally developing superbugs. This experiment evaluated if Manuka honey could be used as an alternative to antibiotics by testing it against an antibiotic‐resistant bacteria. Escherichia coli HB101 K‐12 was transformed to obtain the ampicillin‐resistant gene through the use of pUC19. Manuka honey's effect on antibiotic‐resistant bacteria was evaluated by the Kirby‐Bauer assay alongside antibiotics like ampicillin and streptomycin to determine if Manuka honey produced any significant inhibition of bacterial growth. E. coli was sensitive to all concentrations of Manuka honey but the most effective concentration was 20% w/v with diameters of over 11.00 mm. According to the ANOVA results for the 24 and 48‐hour group 20% w/v was the only concentration that consistently obtained significant results. A minimal inhibitory concentration (MIC) was determined for each treatment to see which antimicrobial compound is the most effective antimicrobial. Streptomycin was the most effective at 64 μg/mL for effectively inhibiting bacterial growth in the 96‐well plate. The study concluded that Manuka honey can be used as an alternative to antibiotics but only in certain concentrations. Support or Funding Information Saint Leo University Biology department funded this project in full.Manuka Honey 20% w/vManuka Honey 20% w/vManuka Honey 30% w/vThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .