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Characterization of glutathione flux and changes in thiol redox balance
Author(s) -
Bandyopadhyay Tirthankar,
Outten Caryn
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.482.2
Subject(s) - glutathione , redox , cytosol , biochemistry , glutathione disulfide , chemistry , mitochondrion , mitochondrial matrix , oxidative phosphorylation , glutaredoxin , thiol , biophysics , microbiology and biotechnology , biology , enzyme , organic chemistry
Maintenance of thiol redox balance is essential in both prokaryotes and eukaryotes. The redox active tripeptide glutathione (GSH) has been shown to be a major cellular redox buffer and is critical for mitochondrial functions such as Fe‐S cluster biogenesis, oxidative phosphorylation and mitochondrial protein import. Since thiol‐disulfide exchange reactions involve the transfer of protons as well as electrons, their redox potentials vary with pH. These pH differences should be taken into consideration while calculating the cytosol, matrix and IMS redox potentials. Previous studies in our group have suggested that GSH:GSSG redox state in subcellular compartments such as the cytosol, mitochondrial matrix and intermembrane space (IMS) is maintained separately [1], but it is still unclear how perturbations of the GSH:GSSG couple influence the distribution of GSH:GSSG between these compartments. Our goal is to understand the mechanisms for maintaining thiol redox balance in different compartments under severe redox stress, using yeast strains overexpressing a plasma membrane GSH:GSSG transporter Hgt1 and deleted in Gsh1 (γ‐glutamylcysteine synthetase, the first enzyme of the glutathione biosynthetic pathway). GSH and GSSG over accumulation were found to directly impact changes in the redox state of the cells as well as thiol redox balance, suggesting concomitant changes in redox potentials as well. Support or Funding Information National Institute of General Medical Sciences (NIH) R35 grant (GM118164)Glutathione flux between subcellular compartmentsThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .