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Study the function of GTPase related genes wasf1 and ect2 in zebrafish vascular development
Author(s) -
Chiu YanHui,
Wu ChangYi
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.477.2
Subject(s) - zebrafish , morpholino , gene knockdown , biology , microbiology and biotechnology , small gtpase , angiogenesis , in situ hybridization , gtpase , model organism , microarray analysis techniques , signal transduction , gene expression , gene , cancer research , genetics
Vascular development is important in vertebrates and regulated by complex signaling pathways. In the process of forming vessels are mainly divided into vasculogenesis and angiogenesis. Zebrafish is a powerful vertebrate model organism to study vascular development. Our previous study in zebrafish identified the transcription factors nr2f1b/islet2 required for blood vessels, mainly focus on the patterning of intersegmental vessels (ISV) and caudal vein plexus (CVP). The further microarray results showed that the expression of several GTPase related genes was regulated by nr2f1b/islet2 , and we are interested in how those GTPase related genes contribute to vascular patterning in zebrafish. In this study, we reported two novel GTPase related genes wasf1 and ect2 that investigated their significance for the vascular development. In‐situ hybridization show that ect2 and wasf1 mRNA is expressed in developing vessels. Knockdown of ect2 and wasf1 by morpholino microinjection cause the defect in blood vessel formation. TUNEL assay and AO staining data show that vascular defects do not caused by cell death, but likely due to the impairment of proliferation and/or migration. Consistent with the defect in vascularture, loss of ect2 or wasf1 reduced the expression of the vascular markers. We further perform Western blotting to examine the relations between wasf1/ ect2 and major angiogentic VEGF/ BMP signals. We showed that GTPase related proteins wasf1 and ect2 play an important roles of vascular development mediated by VEGF/ BMP signalings in zebrafish. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .