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Investigating Alpha‐Arrestin Adaptor Protein Regulation of YOR1 in Yeast
Author(s) -
Cheranda Nina Kiran,
Wang Yuqi
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.476.6
Subject(s) - transporter , saccharomyces cerevisiae , yeast , atp binding cassette transporter , microbiology and biotechnology , signal transducing adaptor protein , arrestin , biology , transport protein , chemistry , biochemistry , g protein coupled receptor , gene , signal transduction
ATP‐binding cassette (ABC) proteins are membrane‐bound transporters that utilize ATP to pump various substrates across biological membranes. Serious pathological conditions can result when these transporters are defective, as exemplified by cystic fibrosis caused by a defective transporter CFTR. Yor1 is an ABC transporter in budding yeast, Saccharomyces cerevisiae. In this study, we investigated the potential regulation of Yor1 by alpha‐arrestins. Using immunoblotting techniques to check the effects of the overexpression of nine different alpha arrestin protein on Yor1 abundance in yeast cells, we found that upregulating the activity of specifically one alpha‐arrestin protein leads to a marked elevation of the level of Yor1. This observation is exciting because it provides a novel strategy for regulating and stabilizing Yor1, which could have valuable implications for the modulation of dysfunctional CFTR transporters in humans with cystic fibrosis. Support or Funding Information Knoedler Undergraduate Fund from Saint Louis University This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .