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Comparison of UCP2 protein expression in normal RIN‐5F and UCP2 inhibited cells
Author(s) -
Willis Jacquelyn,
Clemons Tameka
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.476.35
Subject(s) - amylin , transfection , western blot , mitochondrion , cell culture , intermembrane space , uncoupling protein , oxidative phosphorylation , insulin , protein kinase b , chemistry , medicine , endocrinology , microbiology and biotechnology , phosphorylation , biology , biochemistry , gene , genetics , islet , escherichia coli , bacterial outer membrane , brown adipose tissue , obesity
Uncoupling protein 2 (UCP2) is a mitochondria integral membrane protein that transports protons across the intermembrane space. Traditionally, the function of UCP2 is to impact oxidative phosphorylation by uncoupling ATP synthesis. However, a cell signaling role for UCP2 has recently been investigated. UCP2 expression has been shown to have expression in pancreatic beta cells and the presence of UCP2 has been suggested to lead to a decrease in the release of insulin. Although amylin is co‐secreted with insulin, studies on the relationship between amylin and UCP2 have had little investigation. A known function of amylin is to block glucagon secretion, which leads to a reduction in the rate of glucose production by decreasing the rate of gastric emptying. This study analyzed the protein expression level of amylin in normal RIN‐5F cells and RIN‐5F cells that have been transfected with a UCP2 siRNA construct. The study included stimulating the cells with either 11mM or 30 mM of glucose and comparing the protein expression level in the two cell lines. Western blot analysis was conducted and the results suggest a difference in UCP2 and amylin levels in normal RIN‐5F cells in comparison to the transfected RIN‐5F cells. Support or Funding Information National Science Foundation Research Initiation Award #24430 This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .