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Multilocus Sequence Typing of the Porcine Gastric Pathogen Helicobacter Suis in Nigeria
Author(s) -
Omotosho Oladipo Omotosho,
Jeremiah Olalekan Taiwo,
Emikpe Benjamin O,
Fagbohun Olusegun Adesina,
Odukaye Abayomi Oladapo
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.474.10
Subject(s) - multilocus sequence typing , biology , housekeeping gene , genetics , phylogenetic tree , typing , gene , sequence analysis , microbiology and biotechnology , genotype , gene expression
H. suis is a prevalent cause of gastritis and gastric ulcers in man. The highly fastidious organism mainly colonizes the stomach of domesticated pigs and occasionally jumps to humans. This study was designed to assess the genetic diversity of the porcine H. suis strains in Nigeria. Multilocus sequence typing (MLST) method which is based on the sequencing of the internal gene fragments of seven housekeeping genes ( atpA , efp , mutY , ppa , trpC , ureAB and yphC ) of H. suis was used for direct, culture‐independent strain typing of H. suis obtained from pig stomachs from four locations in Nigeria. Gene fragments ranging from 379 to 732bp and comprising several variable nucleotide sites were sequenced. The sequence type (ST) was defined by the combined allelic profile of the seven alleles. The relationship among the different H. suis strains was examined using the concatenated internal gene fragment sequences of the seven MLST loci. A phylogenetic tree was constructed using the neighbor‐joining method via the PHYLIP 3.69 package, using DNADIST for distance analysis. Three distinct sequence types were assigned and the strains showed close relation to pathogenic human and porcine H. suis strains. The individual pigs were colonized by only 1 H. suis strain. The H. suis strains from Nigeria are g enetically closely related to known pathogenic strains. There is need to ascertain the role of the organism in gastric disease in humans and pigs in Nigeria.Multilocus sequence typing of porcine gastric Helicobacter suis from NigeriaThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .