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Optimization of Protein Extraction and Identification from the European Green Crab
Author(s) -
Wrobel Neal,
Dachel Gabriel,
Shipman Richard,
Myracle Angela D.,
Grant Jennifer E.
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.471.26
Subject(s) - carcinus maenas , protein purification , extraction (chemistry) , chemistry , biology , fishery , biochemistry , chromatography , crustacean , decapoda
Carcinus maenas , or European green crab, is an invasive species off the coast of Maine frequently trapped alongside more desirable crab species, but is too small to provide economic benefits to fishermen. Currently, Marine organisms are widely recognized as intriguing potential sources of peptide antibiotics. Indeed, shells from the green crab may provide an untapped source of protein, potentially providing useful leads for pharmaceutical applications. However, crab shells prose a unique purification problem, in that the hard shells that are rich in hydroxyapatite must first be dissolved. Furthermore, calcium and phosphate must be separated from soluble proteins lest purification strategies result in precipitation of protein‐laden calcium deposits. Here we discuss the optimization of protein extraction protocols, as evidenced by use of the Bradford protein assay and SDS‐PAGE gel analysis. The ultimate goal of this work is to identify novel proteins in these shells by mass spectrometry. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .