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Bioconjugates of Protein A to Gold Nanoparticles
Author(s) -
Lee Christina,
Naing Tiffany,
Herrera Daisy,
Herrera Natalie,
Hoang Audrey,
Marin Melinda
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.461.11
Subject(s) - biomolecule , nanoshell , colloidal gold , nanoparticle , conjugated system , nanotechnology , surface plasmon resonance , materials science , chemistry , biophysics , polymer , composite material , biology
Gold nanoparticles (GNPs) possess rich surface properties with low toxicity that allow it to interact with and participate in conjugation of various biomolecules. As proof of principle, gold nanoparticles were synthesized to a spherical shape with a diameter between 20–30 nm and conjugated to protein A of Staphylococcus aureus bacteria. Conjugation protocols were optimized whereas any size or shaped nanoparticle can be conjugated to any biomolecule. Applications of this technique can be utilized in biomedical imaging techniques to offer more optical contrast and visibility of tagged molecules such as in Magnetic Resonance Imaging (MRI), Chronic Traumatic Encephalopathy (CTE) or to create gold Nano shells in the detection and treatment of cancer cells. GNPs can be synthesized into Nano shells, conjugated to an antibody using the conjugation protocols reported and infused into cancer cells. The gold nanoshells are able to absorb near infrared light, which excites the electrons and, in an attempt, to return to a lower energy, release heat to the external environment. As a result, cancer cells that are also attached receives this heat and are destroyed. The optimization of the nanoparticles to biomolecules will allow for nanomedicines described to become reality. Support or Funding Information NIH BUILD PODER Grant Award Number RL5GM118975 This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .