Premium
Identification of a TetR‐like repressor involved in the regulation of error‐prone DNA polymerases in Acinetobacter baumannii
Author(s) -
Nguyen Brian H,
Macguire Ashley E,
Ching Carly,
Godoy Veronica H
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.458.16
Subject(s) - repressor lexa , tetr , biology , acinetobacter baumannii , dna damage , regulon , genetics , sos response , repressor , gene , dna polymerase , dna , transcription factor , bacteria , pseudomonas aeruginosa
Current knowledge about bacterial DDRs is based off of Escherichia coli , where the global SOS repressor, LexA, controls genes involved in the response to DNA damage. Acinetobacter baumannii is an emerging opportunistic pathogen able to quickly acquire antibiotic resistances and survive desiccation better than other bacteria. Remarkably, A. baumannii does not have a LexA homologue and as a result, there is much to learn about the A. baumannii gene network in response to DNA damage and environmental stress. Clearly, A. baumannii is adept at surviving harsh environments, and we have previously shown that A. baumannii acquires antibiotic resistances due to activities controlled by the DNA damage response (DDR). Moreover, we have evidence suggesting that there are multiple regulators involved in the induction of the A. baumannii DDR and that there are multiple layers involved in its regulation. In this work, we have identified a DDR regulator of error prone DNA polymerases, the DDR‐regulated activities responsible for cellular mutagenesis. Through a forward genetic screen, we have found that when a TetR‐like protein is inactivated, there is deregulation of the expression of several genes that encode error‐prone DNA polymerases and of another previously identified transcription factor, UmuD Ab . Here, we refer to the TetR‐like regulator as EppR ( E rror P rone DNA P olymerase R egulator), and we show that it binds to the promoter region of several genes encoding error‐prone polymerases and that it represses their expression. Our data is consistent with EppR playing a role in the A. baumannii DDR as a repressor of expression of error‐prone DNA polymerases. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .