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Quercetin hydrate promotes osteoblast differentiation and mineralization in vitro
Author(s) -
Wyche Deanna,
Walewska Klaudia,
Belcher Joyce
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.452.17
Subject(s) - osteoblast , alkaline phosphatase , chemistry , viability assay , cell growth , bone cell , microbiology and biotechnology , medicine , biochemistry , cell , in vitro , biology , enzyme
Bone formation by osteoblast cells and osteoclastic bone resorption must be coupled throughout a lifetime to maintain homeostasis of the human skeleton. However as a normal part of the aging process, bone mass begins to decline due to enhanced osteoclasts activity. Osteoblast differentiation progresses through stages of cell proliferation, matrix deposition, and matrix mineralization. The identification of agents, which could regulate bone balance on the cellular level, would have potential therapeutic applications to regulate skeletal disorders. Quercetin hydrate (QH) is a non‐citrus flavonoid that has been previously been studied in its role as an antioxidant. Our study aimed to evaluate the direct effects of QH on osteoblast cell proliferation and differentiation in vitro . QH was added to the pre‐osteoblastic cell lines MC3T3‐E1 and MG‐63 at two concentrations. Cell proliferation and toxicity were assessed at 3 and 7 days of QH treatment via biochemical assays. Cell cultures were differentiated in vitro to 14 and 21 days with osteogenic media and stained to visualize matrix deposition and calcium/phosphate mineral maturation. Alkaline phosphatase was assessed at days 14 and 21 to assess osteoblast enzyme activity and function. Cultures treated with QH showed significant increase in cell proliferation and viability at both treatment doses. In response to QH treatment, there were no toxic effects observed. We observed more mineral matrix staining in QH‐treated cultures compared to untreated control. Von Kossa and Alizarin Red staining demonstrated enhanced osteoblast matrix deposition and mineralization associated with QH treatment. We observed an increase in Alkaline Phosphatase enzyme activity with QH treatment. Collectively, these findings suggest QH has the ability to promote osteoblast cell differentiation, matrix organization and maturation in vitro and could potentially promote skeletal health by acting in a bone anabolic fashion. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .