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Lipopolysaccharide Differentially Alters Oligodendrocyte Precursor Cell Proliferation
Author(s) -
McKinley Raechel Elaine,
Ghaderzadeh Sadaf,
Csoka Antonei
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.451.4
Subject(s) - remyelination , oligodendrocyte , progenitor cell , biology , multiple sclerosis , immunology , tlr4 , microbiology and biotechnology , stem cell , myelin , tlr2 , cell growth , inflammation , neuroscience , cancer research , central nervous system , biochemistry
Multiple Sclerosis (MS) is a debilitating autoimmune disease that impacts 2.3 million people worldwide. In the pathogenesis of the disease, specialized nervous cells called Oligodendrocytes (OLs) are targeted and damaged. When OLs are damaged by the immune system, myelin is destroyed resulting in neuronal cell death. In the adult brain, pools of progenitor cells called Oligodendrocyte Precursor Cells (OPCs) are proliferated, migrated and differentiated to replace and repair mature OLs in the process called remyelination. However, in MS this process does not occur properly. Many factors such as inflammation contribute to the lack of proper remyelination. Moreover, in MS patients, it has been shown that there are increases in Toll‐Like receptors (TLRs). To determine if TLR2 and TLR4 activation plays a role in the proliferation step of remyelination, we treated Induced Pluripotent Stem cell derived OPCs with LPS and hypothesized that LPS treatments will increase the proliferation of OPCs through TLR4 and TLR2 activation. To analyze cell proliferation, a methylthiazol tetrazolium (MTT) assay and immunocytochemistry staining was conducted. Altogether, we found that LPS altered OPC cell proliferation in a dose dependent manner. Support or Funding Information National Institute of Health (NIH) R25 Resource Grant. (1 R25 AG047843‐01) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .