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Using Conformational Penalties to Assess the Structure Dependence of RNA Cellular Function
Author(s) -
AlHashimi Hashim M.
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.220.1
Subject(s) - rna , computational biology , function (biology) , nucleic acid structure , structural biology , biology , biophysics , chemistry , microbiology and biotechnology , genetics , gene
RNA is increasingly recognized as a key player in biology, a novel drug target, and a molecular tool for bioengineering applications and synthetic biology. The degree to which the cellular activity of regulatory RNAs depends on their three dimensional structure can vary from RNA to RNA and can be difficult to determine experimentally. Here, we describe a new approach for assessing the structure dependence of RNA cellular activity that relies on using mutations to introduce conformational equilibria that push the RNA toward non‐native conformations. By comparing the conformational equilibria of these mutants with their impact on cellular activity, we determine the structure‐dependence of cellular function for two highly conserved regulatory elements from HIV‐1. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .