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Method validation of analytical method for determination of extracts of rosemary in various food matrix
Author(s) -
Choi SeungHyun,
Jang GillWoong,
Choi SunIl,
Jung TaeDong,
Cho BongYeon,
Han Xionggao,
Sim WanSup,
Kim YoungCheul,
Lee OkHwan
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb84
Subject(s) - carnosic acid , chromatography , chemistry , rosmarinic acid , food science , detection limit , chromatography detector , matrix (chemical analysis) , high performance liquid chromatography , antioxidant , biochemistry
Extracts of rosemary is a food additive used as antioxidant for edible oil, dressing and sausage. In the EU and China, extracts of rosemary is authorized as food additive with maximum amounts for each food but unauthorized in Korea. Therefore, analytical method to detect extracts of rosemary in food is need. A sensitive simultaneous analytical method to identify rosmarinic acid, carnosol and carnosic acid residues in edible oil, dressing, and sausage was developed and validated using high‐performance liquid chromatography with photo diode array detector (HPLC‐PDA). Validation parameters; specifity, linearity, LOD, LOQ precision and accuracy were attained. The validated method has an analytical range from 1.56 μg/mL to 400 μg/mL in edible oil, dressing and sausage with an LOD and LOQ range from 0.22 to 1.73 μg/mL and 0.66 to 5.23 μg/mL, respectively, depending on the analytes. The recoveries of extracts of rosemary ranged from 70.6 to 116.5% with relative standard deviations between 0.2 and 3.8%. These results showed that the analytical method developed in this study in appropriate for determination of extracts of rosemary identification and can be used to maintain the safety of edible oil, dressing, sausage products containing extracts of rosemary residues. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .