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Hormetic Response of H1299 Proliferation to Extracts of Hydnora Johannis Becc (Kausen Kasa) is Mediated Via Estrogen Receptor/EGFR and PKC
Author(s) -
Morgem Mounira Omar,
Oyagbemi Ademola Adetokunbo,
Omobowale Temidayo Olutayo,
Fagbohun Olusegun,
Adedapo Adeolu Alex,
Yakubu Momoh Audu
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb676
Subject(s) - cell growth , epidermal growth factor receptor , chemistry , estrogen receptor , mtt assay , pharmacology , receptor , calphostin c , tamoxifen , cell culture , cancer research , protein kinase c , estrogen , kinase , medicine , endocrinology , biology , biochemistry , cancer , breast cancer , genetics
Hydnora Johannis Becc (HJ) is a herbal plant used in treating menopausal symptoms and other diseases in different countries, but the mechanism of actions are not known. The effect of HJ (0.1–2 mg/ml) extract on the proliferation of H1299 cell line was investigated. Cells were plated in 96 well plates and the effects of HJ were evaluated using MTT assay. Treatment of H1299 with HJ for 24 hours induced a dose‐dependent increase in cell proliferation increasing growth by 165%, 188%, 203% and 263% at 0.1, 0.25, 0.5 and 2 mg/ml, respectively compared to the control. Paradoxically at 48 hrs, the proliferative effects of the extract were turned into antiproliferative reducing cell growth by −74%, −65%, −37%, −17%, and 108% for 0.1, 0.25, 0.5, 1 and 2 mg/ml, respectively compared to the control. This effects showed a time‐dependent hermetic responses of H1299 to the HJ treatment. To investigate the mechanism(s) by which HJ regulate H1299 growth, cells were treated with HJ in the presence or absence of inhibitors of estrogen receptor (ER; Tamoxifen 10 and 20μg/mL), epidermal growth factor receptor (EGFR; Tyrphostin 5 μM) or Protein Kinase C (PKC; Calphostin 5 μM) and the effects on cell proliferation determined. Inhibition of ER, EGFR, and PKC significantly attenuated the proliferative effects of HJ treatment at 24 hrs. The antiproliferative effects observed at 48 hrs following HJ treatment were reverted to proliferation in the presence of inhibitors. These results suggest that HJ displays paradoxical phenomena which may be mediated by activation/inhibition of both proliferative and apoptotic signaling pathways in H1299 and mediated through interaction with ER, EGFR, and PKC signaling pathways. Further studies are warranted to investigate other signaling pathways involved in the Hydnora Johannis Becc actions. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .