Premium
Arsenic‐Induced Perturbations in Cholinergic System and Energy Metabolism of Rat Brain: Ameliorative effect of Vitamin‐E
Author(s) -
Chinthirla BD,
Konduru KK,
Kozlova EV,
CurrasCollazo MC
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb650
Subject(s) - acetylcholinesterase , chemistry , endocrinology , medicine , aché , neurochemical , atpase , acetylcholine , hippocampus , neurotoxicity , cholinergic , cholinesterase , choline , toxicity , enzyme , biochemistry , biology
Chronic exposure to inorganic arsenic compounds may lead to peripheral and central neurotoxicity. The aim of this study was to examine the impact of Arsenic (As) exposure on cholinergic and bioenergetic systems in three brain regions: cerebral cortex, cerebellum and hippocampus. Adult male Albino rats at 3 months of age were exposed to low (1.5 mg/kg body weight) and high dose As (3 mg/kg body weight) through intraperitoneal injection daily for a period of 3 weeks. A separate batch of rats were given α‐tocopherol (5mg/kg body weight) for a period of one week immediately after the low and high dose As exposure. The acetylcholinesterase (AChE) activity and acetylcholine (ACh) content in synaptosomal fractions and Mg 2+ ‐ATPase and Na + /K + ‐ATPase activities in mitochondrial fractions. We found that As treatment had decreased AChE, Mg 2+ ‐ATPase and Na + /K + ‐ATPase activities and increased in ACh content when compared to control (sham‐injected). The high dose As treated rats showed significantly decreased AChE, Mg 2+ ‐ATPase and Na + /K + ‐ATPase activities and increased ACh content compared to low dose As treated rats. The maximal alteration of these enzymes was observed in hippocampus among the three brain regions. Whereas, the rats supplemented with α‐tocopherol (along with As) showed a reversal effect with slight increase in enzyme activities and decrease in ACh content in a dose dependent manner. In addition to this, As exposure decreased mean body weights in a dose dependent manner. This study demonstrates that exposure to As provoked neurochemical and metabolic disruption by inducing alterations in enzymes of cholinergic system and energy metabolism. Moreover, Vitamin‐E treatment may have partial ameliorative effects on these disturbances caused by As toxicity. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .