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Regulation of Myocardial Ca 2+ Dynamics by the G Protein‐Coupled Estrogen Receptor
Author(s) -
Whitcomb Victoria,
Wauson Eric,
Matnishian Vahe,
Tran QuangKim
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb599
Subject(s) - gper , agonist , estrogen receptor , receptor , extracellular , g protein coupled receptor , chemistry , estrogen , signal transduction , medicine , endocrinology , microbiology and biotechnology , pharmacology , biology , biochemistry , cancer , breast cancer
The G protein‐coupled estrogen receptor (GPER) participates in many cardioprotective activities. However, the mechanisms of such activities are not known. We have begun to investigate the effects of alterations in GPER activity on myocardial signaling via the beta‐1 adrenergic receptor. In freshly isolated primary murine cardiomyocytes, β 1 AR agonist isoproterenol triggers robust Ca 2+ signals that are oscillatory and dependent on extracellular entry. GPER activation dose‐dependently inhibits the isoproterenol‐induced Ca 2+ signals. On the other hand, GPER antagonism exerts the opposite effects. Similar effects were observed in H9C2 cardiomyocytes. Mechanisms of these observations were examined on various components of myocardial Ca 2+ signaling. The data indicate that GPER activity is involved in β 1 AR‐mediated Ca 2+ signaling in the myocardium. Support or Funding Information AHA grant 15SDG25090279 (to E Wauson) and NIH Grant HL112184 (to Q‐K Tran) This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .