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Titanium Dioxide TiO2 Induces Loss of Proliferative Capability Related to Ultrastructural Changes in Human Mesenchymal Stem Cells from Wharton Jelly
Author(s) -
LugoMartínez Gabriela,
GutierrezIglesias Gisela Camelia,
ParraBarrera Alberto,
CalzadaMendoza Claudia Camelia,
RamosGodínez Maria Del Pilar,
CáceresCortés Julio Roberto
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb566
Subject(s) - mesenchymal stem cell , viability assay , wharton's jelly , chemistry , internalization , titanium dioxide , stem cell , anatase , microbiology and biotechnology , biophysics , nanotechnology , cell , biochemistry , materials science , biology , metallurgy , photocatalysis , catalysis
Titanium dioxide is one of the most widely used mineral pigments in the world in the field of paints, cosmetics, sunscreens, plastics, paper, food, etc. It has been reported that TiO 2 at different nanometric scales is considered toxic, depending on the concentration and type of cell that is exposed. TiO 2 nanoparticles (TiO 2 ‐NPs) have the ability to cross biological barriers. In vivo models, it cross the blood‐brain barrier and also it could to cross the fetoplacental barrier, although it is unknown if they are capable to internalize in sites called niches of mesenchymal stem cells, which relevant cells sources due to the importance in regeneration of various tissues. The internalization of nanometric TiO 2 particles on Wharton's jelly hMSC remains unknown. Objective Evaluate the toxic effect and internalization of TiO 2 nanoparticles in hMSCs from Wharton's Jelly. Materials and Methods Wharton gelatin hMSC were cultured and were exposed to three different concentrations (500, 62.5 and 4 μg/mL) of anatase TiO 2 ‐NPs. After 2, 5 and 7 days of exposure, viability tests were performed by alamar blue assay for quantify the toxic effect of TiO 2 . To identify the location of TiO 2 ‐NPs and morphological changes in the hMSCs, various preparations with different TiO 2 concentration were observed by transmission microscopy. Results The results showed that the cellular viability of hMSC decreased significantly at days 2, 5 and 7 of exposure, reduction of viability 40%, 30% and 30% respectively (fig 1). Transmission microscopy showed that the hMSCs had morphological changes and, more specifically, that the TiO 2 ‐NPs took a perinuclear distribution as they accumulated inside the cell, forming a characteristic halo (fig 2). The images showed the internalization of NPs through vesicles, which led to the loss in the continuity of the membrane and the formation of intracytoplasmic spaces representing cellular structural damage in different cellular organelles. Conclusions This results are strong evidences that have to be considered in the regulation of the use of nanotechnology in different products containing TiO 2 without this affecting public health. Sanitary regulation is suggested in the use of TiO 2 ‐NPs to prevent the health condition as it has been done in different countries. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .