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Activation of Growth Signaling in Skeletal Muscle by Growth Arrest Specific‐6
Author(s) -
Wyson Brenna M.,
Matsumura Marc S.,
Deyle Michael R.,
Knowlton M. Nekel,
Arroyo Juan A.,
Hyldahl Robert D.,
Thomson David M.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb493
Subject(s) - protein kinase b , skeletal muscle , gas6 , phosphorylation , mapk/erk pathway , pi3k/akt/mtor pathway , receptor tyrosine kinase , myocyte , microbiology and biotechnology , signal transduction , kinase , c2c12 , biology , medicine , chemistry , endocrinology , myogenesis
Growth‐arrest specific protein 6 (Gas6) is believed to be involved in activation of cell proliferation and growth of many tissues through its activation of the Akt and MAPK signaling pathways downstream of its primary tyrosine kinase receptor, Axl. Currently the role of Gas6 in skeletal muscle has not been elucidated. The purpose of this study was to determine whether skeletal muscle is responsive to Gas6 stimulation. To do this C2C12 myoblasts were incubated in 100 ng/ml of recombinant mouse Gas6 for 15–180 minutes. Gas6 treatment increased Akt phosphorylation, which peaked at 30–60 minutes. We identified Axl expression via immunofluorescence in rat tibialis anterior muscles. Next, soleus (SOL) and plantaris (PLT) muscle strips from 10–12 week old Lewis rats were incubated with or without 200 ng/ml of Gas6 for 30 minutes. Gas6 failed to increase Akt or Erk phosphorylation in slow‐twitch SOL muscles, but increased Erk phosphorylation (but not Akt) in fast‐twitch PLT muscles. We conclude that Gas6 activates Akt or Erk signaling in myoblasts and mature muscle respectively. This suggests that Gas6 may potentially regulate regeneration and/or growth as well as many other cellular processes in skeletal muscle. Support or Funding Information BYU Gerontology Program Grant This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .