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HIGH RESISTANCE OF CHICKEN EMBRYO RED BLOOD CELLS TO ACID ACTION
Author(s) -
Mischenko Alexander,
Lebedeva Elena,
Golovko Vladimir
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb433
Subject(s) - hemolysis , embryo , chemistry , choline , citric acid , andrology , centrifugation , red blood cell , choline chloride , microbiology and biotechnology , biochemistry , biology , immunology , medicine
In this paper, the results of the comparative studies of the resistance of Gallus gallus chicken embryo and human erythrocytes are presented to acid action. Methods Red blood cells from decapitated 10–12 day‐old chicken embryos (5±0.6 g) and human donor blood were used in the experiments. The cells were isolated by centrifugation and washed three times with 145 μmol/l NaCl solution. The hemolysis was performed using: a) Mcllvein buffer solution (MBS) (0.31 g/l Na 2 HPO 4 and 1.87 g/l citric acid, pH = 2.5); b) 0.1 N HCl. The optical density (D) was registered by using photocolorimeter at λ=540 nm. The part of the experiments was made by using isotonic 145 μmol/l choline chloride solution. The experiments were carried out at 18–20°C; the reliability of the differences was evaluated with the Van der Waerden test. Results The hemolytic resistance of the chick embryo erythrocytes was higher than of human erythrocytes. The lag phase value, the time to 50% hemolysis (t 50 ) and the time to complete hemolysis increased twice (p<0.05). The D value remained high after the completion of hemolysis. We believe that the effect of acid on the erythrocyte nucleus led to the decondensation and swelling of chromatin, causing an increase in optical density. The replacement of NaCl with choline chloride did not affect the resistance of human erythrocytes and led to an increase in the chick embryo resistance (p<0.05). In particular, the hemolytic start time (t 0 ) and the end time (t end ) were increased by 16% (p <0.05) and 21% (p <0.05) respectively. The results of these experiments showed that Na+/H+ exchange couldn't lead to an increase in the acid resistance of chick embryo erythrocytes. The calculations indicated that at the anion exchanger density of 2760 copies/μm 2 and 8,300 copies/μm 2 in chicken and human erythrocytes, respectively. The amount of anion exchangers was 1.2 × 10 6 copies per human erythrocyte and 6.3 × 10 5 copies per chick embryo erythrocyte. This is a in good agreement with the 2‐fold increased resistance of the chicken embryo erythrocytes compared to the human erythrocytes. Thus, the high resistance of chicken erythrocytes in comparison with human erythrocytes can be associated with a lower density of anion exchangers in the chicken red blood cells membrane. Support or Funding Information The study was supported by project № AAAA‐A17‐117012310152‐2 This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .