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Effect of Ginger Powder Extracts on Actin Assembly In Vitro
Author(s) -
Sugimoto Naotoshi,
Katakura Masanori,
Matsuzaki Kentaro,
Miyamoto Mayumi,
Sumiyoshi Eri,
Yachie Akihiro,
Shido Osamu
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb420
Subject(s) - mapk/erk pathway , in vitro , p38 mitogen activated protein kinases , fetal bovine serum , lamellipodium , actin , signal transduction , microbiology and biotechnology , in vivo , kinase , chemistry , stimulation , biochemistry , cell migration , biology , endocrinology
Objective The components of ginger root ( Zingiber officinale Roscoe) have been widely used for various medicinal purposes in Asia. Several bioactive compounds have been identified in ginger, including 6‐ and 8‐gingerols and 6‐shogaol, which are agonists of the capsaicin receptor TRPV1 [1, 2] . Recently, we demonstrated that ginger powder might affect human metabolism in vivo [3] . However, the effects of the bioactive compounds of ginger on live cells have not been completely elucidated. In this study, we investigated whether ginger powder extracts (GPE) induced signal transduction, especially actin assembly, in vitro . Method The active components of GPE were characterized by high‐performance liquid chromatography. NIH3T3 mouse fibroblast cells were maintained in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum at 37°C in a 5% CO 2 incubator. These cells were treated with GPE (10 ng/mL) for 10 min, followed by analysis of the activation of mitogen‐activated protein kinases (MAPKs) ERK and p38 MAPK, and actin assembly. Results The levels of phosphorylated ERK and p38 MAPK increased significantly after a 10‐ min stimulation with GPE. In addition, lamellipodia formation, which is known to facilitate cell migration [4] , occurred at cell edges after GPE stimulation. Conclusions GPE activated MAPKs and increased lamellipodia formation in mouse fibroblasts. These results suggest that GPE modifies signal transduction, including actin assembly, in vitro . Support or Funding Information KAKENHI, Japan This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .