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The effects of starch‐entrapped microsphere supplementation on obesity and insulin resistance in C57BL/6J mice
Author(s) -
McNabney Sean M,
Reed Kylee M,
Starrs Grace E,
Libbey Margaret E,
Henagan Tara M
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb375
Subject(s) - resistant starch , insulin resistance , butyrate , medicine , endocrinology , obesity , starch , chemistry , insulin , glucose tolerance test , food science , fermentation
Dietary supplementation with the short chain fatty acid butyrate prevents obesity and insulin resistance in mice fed a high fat diet (HFD). Unfortunately, the translational potential of dietary butyrate supplementation is limited due to its teratogenic effects at higher doses. More recently, attention has turned to resistant starches that produce butyrate during their fermentation in the colon as therapeutic agents for attenuating obesity and insulin resistance. These starches represent an effective strategy to enhance endogenous butyrate production. The entrapment of resistant starch in an alginate matrix represents a novel technique to increase fermentable carbohydrate delivery to the colon. The purpose of this study was to assess the effects of starch‐entrapped microsphere (SM) supplementation on body composition, insulin sensitivity and energy expenditure in a murine model of diet‐induced obesity. Male C57BL/6J mice were fed the following diets (N=10/group): 1) low fat diet (LF, 10% kcal fat), 2) HFD (HF, 60% kcal fat), or 3) HFD + 10% wt/wt SM (HF+SM, 60% kcal fat). Body weight and 48 h food consumption were measured weekly. Body composition was assessed weekly using EchoMRI, with scans performed in duplicate. Glucose tolerance tests (N=5/group) were performed after 7 wks of feeding and area under the curve (AUC) was determined. Energy expenditure (N=5/group) was assessed via CLAMS at 9 wks of feeding. All data were analyzed via repeated or one‐way ANOVA and post hoc Tukey tests as appropriate. Body weight, percent fat mass and GTT AUC was significantly greater in HF compared to LF. Energy expenditure and respiratory exchange ratio (RER) were significantly decreased in HF compared to LF; whereas, caloric consumption was increased. Although HF+SM showed significantly increased body weight and percent fat mass relative to LF, both body weight and percent fat mass were significantly decreased compared to HF. There was no significant difference in GTT AUC between LF and HF+SM. HF+SM showed significantly decreased GTT AUC compared to HF. Energy expenditure in HF+SM was not different from LF and was significantly increased compared to HF. RER was significantly decreased in HF+SM compared to LF and not different between HF and HF+SM. Interestingly, food consumption was significantly increased in HF+SM compared to both LF and HF groups. Collectively, our data show that dietary SM supplementation attenuates HFDinduced obesity and insulin resistance through upregulation of whole body energy expenditure, despite increased caloric intake, and may be an effective treatment strategy for obesity and insulin resistance. Support or Funding Information This work was supported by a AgSeed grant to TMH. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .