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Correlation between Promoter CpG Methylation and mRNA Levels of Genes Regulated by Fatty Acids in Muscle Cells and Adipocytes
Author(s) -
Alam Md Masudul,
Cheng Elliott,
Li Yi
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb367
Subject(s) - dna methylation , epigenetics , methylation , gene , cpg site , biology , bisulfite sequencing , dna microarray , microbiology and biotechnology , gene expression , adipose tissue , genomic dna , microarray , genetics , endocrinology
In obesity and type 2 diabetes, adipose and muscle tissues are significantly affected. It has been demonstrated that many of the changes are associated with gene expression. We tried to study the involvement of epigenetic modifications in mediating gene expression induced by hyperlipidemia. L6 Muscle cells and differentiated 3T3L1 adipocytes were treated with saturated and polyunsaturated fatty acids to mimic hyperlipidemia condition. After treatments, methylation sequencing was done to identify the methylated regions of the genomic DNA samples from the cells. In combination with validated DNA microarray data, we have found an inverse correlation between DNA CpG methylation in the promoter region and the mRNA level of a number of genes including FADS3, PPARγ, RASA2, MEF2C. At present we are individually validating the methylation status of the genes and studying the signaling pathways mediating in methylation of these genes. Support or Funding Information This research is supported by grant SC3GM116737 from the NIGMS of the NIH to Y. Li. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .