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Potential Role of Structural Variants of Circulating Lipopolysaccharide in Exercise‐Induced Heat Illness
Author(s) -
Wright Skylar,
Hosokawa Yuri,
Dadzie Ekow C,
Lopez Virgilio,
Colburn Abigail T,
Bivona Joseph J,
Thorton Staci N,
Adams William M,
Brown Cyrus,
Katch Rachel K,
Huggins Robert A,
Stearns Rebecca L,
Jardine John F,
Davis Robert J,
Casa Douglas J,
Lee Elaine C
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb258
Subject(s) - lipopolysaccharide , microbiome , immune system , immunology , inflammation , sepsis , chemistry , biology , bioinformatics
Previous investigations at the Falmouth Road Race (FRR; Falmouth, MA) indicated that combined exercise, heat, and dehydration stress increased gastrointestinal tract permeability. This subsequently exposed the circulatory system to components of the microbiome usually found only in the gut. Lipopolysaccharide (LPS), a component of Gram‐negative bacterial membranes, is one such element released into circulation, especially post‐exercise. LPS in circulation activates host immune cell signaling cascades that can lead to inflammatory responses (e.g. cell pyroptosis, exertional heat stroke, and sepsis) and upregulated proinflammtory gene expression (e.g. TNF‐ α and IL‐1 β ). While subjects of the 2015 FRR (N=30) had elevated core temperature post race compared to pre (pre 36.87°C vs. post 39.87°C, p<0.05), there is unexplained variation in the relationship among the level of circulating LPS (2.90EU/ml pre vs. 3.48EU/ml post, p<0.05; Hycult Biotech, ELISA), core temperature, and subsequent heat stroke presentation. Since some strains of bacteria induce a strong immune response while others do not, it is of interest to determine if structural variations in LPS contribute to varying immune system activation during exercise‐heat stress. Diet influences the composition of the microbiome and different microbiome species have varying LPS structures. Because of this, even short‐term alterations in macronutrient consumption can alter the composition and gene expression of the microbiome. Ongoing research will utilize extraction of LPS from pre‐race and post‐race plasma and HPLC/MS to characterize structural variations in LPS in individuals of the 2015, 2016, and 2017 FRR. Support or Funding Information New investigator start‐up, McNair Scholar Program, FRR This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .