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Effect of Age and Acute Exercise on Circulating Endostatin
Author(s) -
Temperley McKenzie K.,
Mardis Benjamin R.,
Iwamoto Erika,
Bock Joshua M,
Casey Darren P.,
Luttrell Meredith
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb253
Subject(s) - endostatin , medicine , venous blood , endocrinology , heart rate , ageing , blood pressure , vegf receptors
BACKGROUND Endostatin may have both angiogenic and angiostatic effects in vivo, although angiostatic effects appear to predominate. It has been reported that circulating levels of endostatin increase after acute exercise in young adults; however, it is unclear whether healthy older adults exhibit a similar response. PURPOSE The purpose of this study was to determine the effect of age on changes in serum endostatin concentration following an acute bout of dynamic exercise. METHODS Ten young (25 ± 1 years, 5M/5F) and nine older (68 ± 2 years, 4M, 5F) healthy but sedentary to recreationally active adults completed a single 45‐minute bout of submaximal cycle ergometer exercise at 60 – 65% of their age‐predicted maximal heart rate. Venous blood samples were obtained prior to (PREX), immediately post‐ (POSTX0), and at three hours post‐exercise (POSTX3). Serum concentrations of endostatin were analyzed at each time point using an enzyme‐linked immunosorbent assay. Absolute protein concentrations at each time point and fold‐change from PREX concentrations are reported. RESULTS There was no difference in PREX serum endostatin concentration between young (136.2 ± 18.48 pg/mL) and older adults (125.9 ± 11.48 pg/mL) (p=0.81). Additionally, there was no age effect on absolute endostatin concentrations at POSTX0 and POSTX3 ( p = 0.06 for interaction). However, when comparing the fold‐change in endostatin concentration after exercise, there was a significant interaction (p = 0.01) between age and POSTX0 to POSTX3 time point. The main effects (time, age) were not significant. Simple main effects analysis revealed a difference in fold‐change in concentration of endostatin from PREX in older (p = 0.02) but not younger (p = 0.80) adults from POSTX0 to POSTX3. CONCLUSIONS Our data suggests there is no difference in either PREX serum endostatin concentrations between young and older adults. However, older adults may have a different temporal response in serum endostatin following a single bout of moderate intensity dynamic exercise compared to young adults. Support or Funding Information Research Support: University of Iowa Carver College of Medicine FUTURE in Biomedicine Program (MJL, BRM, DPC). This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .