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Effects of Acute Aerobic Exercise on Transcriptomics in Skeletal Muscle of Lean vs Overweight/Obese Men
Author(s) -
Devarshi Prasad P,
Jones Aarin D,
Taylor Erin M,
Henagan Tara M
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb248
Subject(s) - overweight , skeletal muscle , aerobic exercise , lean body mass , endocrinology , medicine , transcriptome , vastus lateralis muscle , gene expression , gene , biology , obesity , genetics , body weight
Aerobic exercise improves skeletal muscle mitochondrial function and improved insulin sensitivity in lean and overweight and obese (Ov/Ob) individuals; however, the genes important in determining the beneficial effects of exercise in both lean and Ov/Ob individuals are still largely unknown. Here, we aimed to identify genes similarly expressed in lean and Ov/Ob skeletal muscle following an acute bout of exercise. 30 men, aged 19–30 years, were recruited and divided into two groups: lean (BMI<25, 18.5–24.1 kg/m2, n=15) and Ov/Ob (BMI≥25, 25.5–36.9 kg/m2, n=15). Four hours after eating a standardized high‐carbohydrate breakfast (7 kcal/kg; 60% carbohydrate, 25% fat, 15% protein), participants performed an acute bout of cycling exercise (50% VO2max, spending ~650 kcal). Vastus lateralis muscle biopsy samples were collected prior to (Pre) and immediately following (Post) the acute exercise bout. In skeletal muscle, gene expression by RNA‐seq was determined using the Illumina platform. For analysis, TopHat was used for sequence alignment and Cuffdiff for differential gene expression analysis with FDR set at 0.05. ~97% of all sequenced reads from RNA‐seq aligned to the genome. Gene Ontology analysis was performed using DAVID. Analysis showed that 297 genes were differentially regulated between lean Pre and Ov/Ob Pre, 1230 genes between lean Pre and lean Post, 1123 genes between Ov/Ob Pre and Ov/Ob Post and 161 between lean Post and Ov/Ob Post. In both lean and Ov/Ob, genes upregulated following exercise were enriched for GO terms related to lipid metabolism, mitochondrial biogenesis/function and insulin signaling. Pathway analysis revealed that the AMP‐activated protein kinase (AMPK) signaling pathway was one of the pathways enriched in both lean Post and Ov/Ob Post, with 11 genes in the pathway upregulated in lean Post and 9 in Ov/Ob Post. In conclusion, it is possible that similar expression of genes following exercise may account for the increased responsiveness in mitochondrial function and insulin signaling in both lean and Ov/Ob. Support or Funding Information This work was supported by Ralph W. and Grace M. Showalter Research Trust grant and Indiana CTSI funds to TMH. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .