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Expanding the Horizon of Recombination Repair in Mycobacteria: Identification and Characterization of Novel Proteins
Author(s) -
Singh Amandeep
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.lb1
Subject(s) - homologous recombination , dna repair , dna , dna damage , gene , recombination , single stranded binding protein , dna mismatch repair , chemistry , mycobacterium smegmatis , genetics , biology , dna binding protein , microbiology and biotechnology , computational biology , transcription factor , mycobacterium tuberculosis , tuberculosis , medicine , pathology
In prokaryotes, repair by homologous recombination provides a major means to reinstate the genetic information lost in DNA damage. Recent research on recombination repair in mycobacteria has led to significant revision of the understanding of this system. A comparative analysis of the system, carried out in 43 mycobacterial genomes, lead to identification of novel proteins. To substantiate the predictions, biochemical and structural investigations on probable roles of two proteins, namely, SSBb and RecG wed , were carried out. The structure of M. smegmatis SSBb ( Ms SSBb), was determined at 2.5 □ resolution. Solution studies indicate that the canonical SSB ( Ms SSBa) is more stable towards thermal and chemical denaturation than Ms SSBb. Also, Ms SSBa has a two‐fold higher DNA binding affinity than Ms SSBb. A direct physical interaction between Ms SSBb and Ms RecA was established, suggesting a role during recombination. The expression levels of ssbB gene increased by approximately two‐ and sevenfold in UV and hypoxic stress, respectively, while concurrently the levels of ssbA expression declined. These results indicate a role of Ms SSBb in recombination repair during stress. Ms RecG wed binds a variety of branched DNA structures in vitro , while it does not bind ss‐ or dsDNA. The up‐regulation of recG wed during UV damage, and down‐regulation during H 2 O 2 /MMS stress, in M. smegmatis indicated a possible role in post‐replicative recombination events that proceed though branched DNA intermediates. Taken together, this work augments the understanding of the repertoire of proteins known to be involved in DNA repair pathways in mycobacteria. Support or Funding Information Department of Biotechnology, India This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .