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Bio‐inspired micropattern modulates stem cell fate and directs well‐ordered tubular tissue formation
Author(s) -
Liu Xiaohua
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.94.3
Subject(s) - micropatterning , regeneration (biology) , nanotechnology , stem cell , materials science , microbiology and biotechnology , biology
Many tissues in the body possess highly‐organized microstructures that are a prerequisite for their physiological functions. However, regeneration of the tissues with well‐ordered microstructures has long been a challenge due to unprecedented technology difficulties. Here, we report a unique maskless micropatterning technology to create bio‐inspired nanofibrous 3D matrices with highly‐organized microstructure for tubular tissue regeneration. Our micropatterning method is a laser‐guided, non‐contact, high‐precision, flexible computer programming of machining process that creates highly‐ordered tubules (2–5 μm) in the bio‐inspired 3D matrix. The tubular architecture presents pivotal biophysical cues to control dental pulp stem cell alignment, migration, polarization, and differentiation. More importantly, when using this hierarchical matrix as a template, we successfully regenerated functional tubular dentin (the main component of a tooth) in vivo. Our bio‐inspired micropattern approach represents a powerful avenue not only for the exploration of a range of fundamental biological questions, but also for the regeneration of functional tissues with well‐organized structures. Support or Funding Information This research is supported by NIH/NIDCR R01DE024979. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .