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Alcohol‐Induced Gut Lymphatic Leak Results in Adipocentric Immunometabolic Dysregulation in Female Rats
Author(s) -
SouzaSmith Flavia M.,
Simon Liz,
Siggins Robert,
Molina Patricia E.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.924.6
Subject(s) - lymphatic system , insulin resistance , immune system , flow cytometry , immunology , t cell , endocrinology , medicine , inflammation , immune dysregulation , adipose tissue , biology , insulin
Previously we demonstrated that alcohol induces mesenteric lymphatic vessel (MLV)hyperpermeability, perilymphatic adipose tissue (PLAT) inflammation, and impaired insulin signaling in male rats. MLV play an important role in the trafficking of gut mucosal derived dendritic cells (DC) to mesenteric lymph nodes (MLN). Leakage of DC from MLV has been reported in infectious states. DC promote regulatory T cell (Treg) expansion in adipose tissue and this mechanism has been proposed to be a driver of age‐associated insulin resistance. We hypothesize that alcohol‐induced MLV hyperperemeability results in DC leakage to PLAT and this contributes to immunometabolicdys function in chronic alcohol fed animals. To test this hypothesis, female Fisher 344 rats were randomized to Lieber‐DeCarli liquid diet (36% of calories from alcohol) for 10 weeks or pair‐fed control groups. PLAT, MLN, and peripheral blood lymphocytes (PBL) were isolated for phenotyping by flow cytometry. Alcohol‐fedanimals had increased PLAT Tregs and CD4 T cells and decreased % Tregs in PBLs. These findings suggest that alcohol‐induced lymphatic leakage is associated with DC deviation into PLAT that we speculate mediates Treg expansion and initiatesmetabolic dysregulation. In conjunction with our previous findings, these results support an alcohol‐induced immune cell deviation from the gutlymphnode pathway that promotes PLAT immunometabolic dysregulation and couldpotentially have additional implications on induction of mucosal immunity. Support or Funding Information Supported by LSUHSC Department of Physiology. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .